Peptide Calculator

Peptide Dosage Calculator

Correct Peptide Reconstitution:

Peptides are delicate biomolecules that require precise handling and storage to maintain their integrity and effectiveness. Our peptides are delivered as lyophilized (freeze-dried) powders, which offer stability against short-term temperature fluctuations during transit. Even at room temperature, these powdered peptides remain stable for several weeks. However, to maximize their shelf life and ensure consistent results, proper storage and reconstitution methods must be followed.

Storage and Handling Best Practices

Before Reconstitution: Store lyophilized peptides in a dry, cool place, preferably at -20°C to -80°C for long-term preservation. This prevents degradation and contamination.

After Reconstitution: Once peptides are reconstituted, they become more susceptible to degradation. Store solutions at 4°C for short-term use (a few days) or freeze aliquots at -20°C for longer periods. Avoid repeated freeze-thaw cycles, as these can reduce the peptide's bioactivity.

How to Reconstitute Peptides Properly

Choose the Right Solvent: The solvent choice depends on the peptide’s solubility.

  1. Suitable for many peptides but may not dissolve hydrophobic peptides completely.Acetic acid (0.1%) or dilute HCl.
  2. Can be used sparingly for highly hydrophobic peptides.
  3. Used when specific pH conditions are required.

Gently Add the Solvent:

Check for Clarity: Ensure the solution is clear and free of undissolved particles. If needed, sonication (brief exposure to ultrasonic waves) can help dissolve stubborn peptides.

Common Mistakes to Avoid

  1. Using tap or non-sterile water, which may introduce contaminants.
  2. Shaking the vial aggressively, which can degrade the peptide.
  3. Allowing the reconstituted solution to sit at room temperature for extended periods.
  4. Not aliquoting solutions for storage, leading to repeated freeze-thaw cycles.

By following these guidelines, researchers can ensure that peptides retain their potency and remain viable for various experimental applications. Proper reconstitution and storage practices contribute significantly to the accuracy and reliability of research results.